| First Author | Douros JD | Year | 2018 |
| Journal | Diabetes | Volume | 67 |
| Issue | 8 | Pages | 1504-1511 |
| PubMed ID | 29759973 | Mgi Jnum | J:264032 |
| Mgi Id | MGI:6192425 | Doi | 10.2337/db18-0081 |
| Citation | Douros JD, et al. (2018) Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a beta-Cell Glucagon-Like Peptide 1 Receptor. Diabetes 67(8):1504-1511 |
| abstractText | Bariatric surgeries, including vertical sleeve gastrectomy (VSG), resolve diabetes in 40-50% of patients. Studies examining the molecular mechanisms underlying this effect have centered on the role of the insulinotropic glucagon-like peptide 1 (GLP-1), in great part because of the approximately 10-fold rise in its circulating levels after surgery. However, there is currently debate over the role of direct beta-cell signaling by GLP-1 to mediate improved glucose tolerance following surgery. In order to assess the importance of beta-cell GLP-1 receptor (GLP-1R) for improving glucose control after VSG, a mouse model of this procedure was developed and combined with a genetically modified mouse line allowing an inducible, beta-cell-specific Glp1r knockdown (Glp1r(beta-cell-ko)). Mice with VSG lost approximately 20% of body weight over 30 days compared with sham-operated controls and had a approximately 60% improvement in glucose tolerance. Isolated islets from VSG mice had significantly greater insulin responses to glucose than controls. Glp1r knockdown in beta-cells caused glucose intolerance in diet-induced obese mice compared with obese controls, but VSG improved glycemic profiles to similar levels during oral and intraperitoneal glucose challenges in Glp1r(beta-cell-ko) and Glp1r(WT) mice. Therefore, even though the beta-cell GLP-1R seems to be important for maintaining glucose tolerance in obese mice, in these experiments it is dispensable for the improvement in glucose tolerance after VSG. Moreover, the metabolic physiology activated by VSG can overcome the deficits in glucose regulation caused by lack of beta-cell GLP-1 signaling in obesity. |
