| First Author | Thakar K | Year | 2012 |
| Journal | FEBS J | Volume | 279 |
| Issue | 5 | Pages | 737-51 |
| PubMed ID | 22212508 | Mgi Jnum | J:194138 |
| Mgi Id | MGI:5470382 | Doi | 10.1111/j.1742-4658.2011.08464.x |
| Citation | Thakar K, et al. (2012) Interaction of HRP-2 isoforms with HDGF: chromatin binding of a specific heteromer. FEBS J 279(5):737-51 |
| abstractText | Hepatoma-derived growth-factor-related protein 2 (HRP-2) belongs to a family with five additional members: hepatoma-derived growth factor (HDGF); lens epithelium-derived growth factor; and the HDGF-related proteins -1, -3 and -4. Very little is known regarding the function of HRP-2 in particular. This study shows for the first time heteromer formation of different members of the HRP family; HDGF and HRP-2. In addition, we discovered a previously unknown splice variant of HRP-2 mRNA encoding for a protein with a 53-amino acid deletion in its hath region. This HRP-2 isoform c interacts preferentially with a processed form of HDGF probably because of the loss of an alpha helix of HRP-2. Furthermore, in contrast to other isoforms of HRP-2, isoform c binds to chromatin similar to its most closely related family member lens epithelium-derived growth factor with potential consequences regarding its function in HIV integration. Interestingly, only the new HRP-2 isoform c and a processed form of HDGF are displaced from condensed mitotic metaphase chromatin. In conclusion, these observations provide a new perspective for understanding the biological functions of HDGF and related proteins. |
