| First Author | Nakamura M | Year | 1996 |
| Journal | Biochim Biophys Acta | Volume | 1284 |
| Issue | 2 | Pages | 134-7 |
| PubMed ID | 8914576 | Mgi Jnum | J:36684 |
| Mgi Id | MGI:84110 | Doi | 10.1016/s0005-2736(96)00166-6 |
| Citation | Nakamura M, et al. (1996) Cloning and functional expression of a cDNA encoding a mouse type 2 neuropeptide Y receptor. Biochim Biophys Acta 1284(2):134-7 |
| abstractText | A cDNA clone homologous with the human neuropeptide Y (NPY)-Y2 receptor has been isolated from a mouse brain cDNA library. Analysis of the predicted amino-acid sequence indicates that the polypeptide encoded by this cDNA is 94% homologous to the human NPY-Y2 receptor. In Chinese hamster ovary (CHO) cells expressing the mouse NPY-Y2 receptor, an increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were observed due to stimulation with NPY, NPY-(13-36) and peptide YY, but not with pancreatic polypeptide or [Leu31, Pro34]NPY. The fact that the NPY-induced increase in intracellular Ca2+ and inhibition of forskolin-induced cAMP accumulation were eliminated by pretreatment with pertussis toxin suggests that the NPY-Y2 receptor couples to PTX-sensitive G-protein(s), probably Gi/Go, in CHO cells. |
