| First Author | Cartagena-Rivera AX | Year | 2019 |
| Journal | Sci Adv | Volume | 5 |
| Issue | 2 | Pages | eaat9934 |
| PubMed ID | 30801007 | Mgi Jnum | J:288364 |
| Mgi Id | MGI:6431964 | Doi | 10.1126/sciadv.aat9934 |
| Citation | Cartagena-Rivera AX, et al. (2019) Cochlear outer hair cell horizontal top connectors mediate mature stereocilia bundle mechanics. Sci Adv 5(2):eaat9934 |
| abstractText | Outer hair cell (OHC) stereocilia bundle deflection opens mechanoelectrical transduction channels at the tips of the stereocilia from the middle and short rows, while bundle cohesion is maintained owing to the presence of horizontal top connectors. Here, we used a quantitative noncontact atomic force microscopy method to investigate stereocilia bundle stiffness and damping, when stimulated at acoustic frequencies and nanometer distances from the bundle. Stereocilia bundle mechanics were determined in stereocilin-deficient mice lacking top connectors and with detached tectorial membrane (Strc (-/-)/Tecta (-/-) double knockout) and heterozygous littermate controls (Strc (+/-)/Tecta (-/-)). A substantial decrease in bundle stiffness and damping by ~60 and ~74% on postnatal days P13 to P15 was observed when top connectors were absent. Additionally, we followed bundle mechanics during OHC top connectors development between P9 and P15 and quantified the observed increase in OHC bundle stiffness and damping in Strc (+/-)/Tecta (-/-) mice while no significant change was detected in Strc (-/-)/Tecta (-/-) animals. |
