| Primary Identifier | MGI:5306956 | Allele Type | Targeted |
| Attribute String | Hypomorph, Inserted expressed sequence | Gene | Sirt1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A C to T mutation resulting in a histidine to tyrosine substitution at codon 355 (H355Y) was introduced in exon 5 and a floxed hygromycin/thymidine kinase fusion gene with splice acceptor and donor sequences was inserted upstream of exon 5 via homologous recombination. Cre mediated recombination removed the hygromycin/thymidine kinase fusion gene. The mutant protein is expressed at levels similar to the wild-type protein. The mutant protein lacks acetylation activity in MEFs. |
