| First Author | Yonezawa S | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 271 |
| Issue | 2 | Pages | 526-33 |
| PubMed ID | 10799329 | Mgi Jnum | J:62180 |
| Mgi Id | MGI:1858545 | Doi | 10.1006/bbrc.2000.2669 |
| Citation | Yonezawa S, et al. (2000) Mouse myosin X: molecular architecture and tissue expression as revealed by northern blot and in situ hybridization analyses. Biochem Biophys Res Commun 271(2):526-33 |
| abstractText | The structure of the coding region of mouse myosin X cDNA was determined. The predicted protein sequence indicated an approximately 240 kDa molecular mass with 2062 amino acids. When aligned with the structure predicted for calf myosin X (GenBank Accession No. U55042), extremely highly conserved pleckstrin homology domains and a myosin tail homology 4 domain were apparent in the tail region, suggesting their importance for myosin X's function. Northern blot analysis revealed the existence of a myosin X mRNA, 8.7 kb in size, in various mouse tissues, while a similar size of human type myosin X mRNA was recognized mainly in the testis. In addition to the adult-type transcripts in mice, a smaller embryo-specific mRNA, 4.8 kb in size, was identified in early to late embryonic stages, suggesting the presence of a shorter myosin X isoform in mouse embryos. In situ hybridization experiments with mouse testis revealed that myosin X mRNA was restricted to Sertoli cells at stages VIII-X of the spermatogenesis cycle, suggesting that myosin X is implicated in the supporting cells during the spermatid morphogenesis. Copyright 2000 Academic Press. |
