| First Author | Manzke T | Year | 2010 |
| Journal | J Clin Invest | Volume | 120 |
| Issue | 11 | Pages | 4118-28 |
| PubMed ID | 20978350 | Mgi Jnum | J:166914 |
| Mgi Id | MGI:4850195 | Doi | 10.1172/JCI43029 |
| Citation | Manzke T, et al. (2010) Serotonin receptor 1A-modulated phosphorylation of glycine receptor alpha3 controls breathing in mice. J Clin Invest 120(11):4118-28 |
| abstractText | Rhythmic breathing movements originate from a dispersed neuronal network in the medulla and pons. Here, we demonstrate that rhythmic activity of this respiratory network is affected by the phosphorylation status of the inhibitory glycine receptor alpha3 subtype (GlyRalpha3), which controls glutamatergic and glycinergic neuronal discharges, subject to serotonergic modulation. Serotonin receptor type 1A-specific (5-HTR1A-specific) modulation directly induced dephosphorylation of GlyRalpha3 receptors, which augmented inhibitory glycine-activated chloride currents in HEK293 cells coexpressing 5-HTR1A and GlyRalpha3. The 5-HTR1A-GlyRalpha3 signaling pathway was distinct from opioid receptor signaling and efficiently counteracted opioid-induced depression of breathing and consequential apnea in mice. Paradoxically, this rescue of breathing originated from enhanced glycinergic synaptic inhibition of glutamatergic and glycinergic neurons and caused disinhibition of their target neurons. Together, these effects changed respiratory phase alternations and ensured rhythmic breathing in vivo. GlyRalpha3-deficient mice had an irregular respiratory rhythm under baseline conditions, and systemic 5-HTR1A activation failed to remedy opioid-induced respiratory depression in these mice. Delineation of this 5-HTR1A-GlyRalpha3 signaling pathway offers a mechanistic basis for pharmacological treatment of opioid-induced apnea and other breathing disturbances caused by disorders of inhibitory synaptic transmission, such as hyperekplexia, hypoxia/ischemia, and brainstem infarction. |
