First Author | Schlegel W | Year | 1996 |
Journal | Gene | Volume | 178 |
Issue | 1-2 | Pages | 115-8 |
PubMed ID | 8921901 | Mgi Jnum | J:36429 |
Mgi Id | MGI:83859 | Doi | 10.1016/0378-1119(96)00347-2 |
Citation | Schlegel W, et al. (1996) Cloning and analysis of a cDNA encoding the BALB/c murine erythrocyte band 7 integral membrane protein. Gene 178(1-2):115-8 |
abstractText | cDNA clones encoding the BALB/c murine erythrocyte band 7 integral membrane protein (also termed protein 7.2b, or 'stomatin') were isolated by the screening of a corresponding bone-marrow lambda gt11 cDNA library with a human cDNA probe, and by 5'-RACE PCR cloning. Comparison of the murine, human and Caenorhabditis elegans protein 7.2b amino acid (aa) sequences revealed overall identities of 88% (human) and 61% (C. elegans), with the N-terminal domains showing only little similarity. The 7.2b protein sequences of the two mouse strains, BALB/c and C57BL/6J (B6), showed six rather conservative aa substitutions, three of them in the hydrophobic domain. The BALB/c murine mRNA, about 3.5 kb in size, is widely expressed in various tissues, most notably in spleen, lung and testis. |