| First Author | Li ZX | Year | 2009 |
| Journal | Neurosci Lett | Volume | 465 |
| Issue | 2 | Pages | 128-32 |
| PubMed ID | 19732808 | Mgi Jnum | J:155501 |
| Mgi Id | MGI:4414618 | Doi | 10.1016/j.neulet.2009.08.069 |
| Citation | Li ZX, et al. (2009) Granulocyte-colony stimulating factor is involved in low-dose LPS-induced neuroprotection. Neurosci Lett 465(2):128-32 |
| abstractText | Granulocyte-colony stimulating factor (G-CSF) has recently been noted for neuroprotective function. Evidence has been given to indicate that G-CSF is naturally expressed in neurons and directly activates anti-apoptosis pathways. Finding out the agents inducing G-CSF production is of value for understanding the neuroprotection network in central nervous system. It is known that lipopolysaccharide (LPS) can induce macrophages to produce G-CSF. Here we demonstrate that hippocampal neurons exhibited the expression of toll-like receptor-4, and prove that low-dose LPS treatment increased the expression and production of G-CSF mRNA and protein in cultured neurons. We further indicate that the neutralization of G-CSF with corresponding anti-G-CSF antibodies abolished the neuroprotective effect of LPS pretreatment in N-methyl-D-aspartic acid-induced neuronal injury by MTT/CCK-8 assays and LDH release. Thus our results reveal that G-CSF may be involved in LPS-mediated neuroprotection in vivo. |
