| First Author | Ellinghaus P | Year | 1998 |
| Journal | Biochim Biophys Acta | Volume | 1397 |
| Issue | 2 | Pages | 175-9 |
| PubMed ID | 9565681 | Mgi Jnum | J:47197 |
| Mgi Id | MGI:1202741 | Doi | 10.1016/s0167-4781(98)00023-2 |
| Citation | Ellinghaus P, et al. (1998) Cloning and sequencing of a novel murine liver carboxylesterase cDNA. Biochim Biophys Acta 1397(2):175-9 |
| abstractText | Carboxylesterases (EC 3.1.1.1) comprise a group of serine hydrolases with at least 20 genetically distinct loci in mice. Here, we describe differential display PCR-based cloning of a cDNA, encoding a novel murine carboxylesterase termed ES-x, which was expressed predominantly in liver but also in kidney and lung. The cDNA of ES-x spanned a 2249-bp sequence with an open reading frame encoding 565 amino acids, including an N-terminal hydrophobic signal peptide which directs the synthesis into microsomal lumen and a C-terminal HVEL consensus sequence for retaining the protein in the lumen of the endoplasmic reticulum. The predicted amino acid sequence of ES-x exhibited 75% identity with rat liver pI 6.1 esterase. We further demonstrate that feeding mice with diets containing cholestyramine or sodium cholate increases mRNA-expression of ES-x in liver 2.5- to 3-fold. Copyright 1998 Elsevier Science B.V. |
