| First Author | Rehli M | Year | 1996 |
| Journal | Biochem Biophys Res Commun | Volume | 226 |
| Issue | 2 | Pages | 420-5 |
| PubMed ID | 8806650 | Mgi Jnum | J:35261 |
| Mgi Id | MGI:82713 | Doi | 10.1006/bbrc.1996.1371 |
| Citation | Rehli M, et al. (1996) Molecular cloning and expression of mouse procalcitonin. Biochem Biophys Res Commun 226(2):420-5 |
| abstractText | The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify calcitonin cDNA as 5'-end and 3'-end fragments from mouse thyroid RNA. The obtained cDNA is 850 bp in length most probably representing the entire mouse calcitonin mRNA. It contains an open reading frame coding for a 136 amino acid protein with a calculated M(r) of 15,143. Comparison of the deduced amino acid sequences of preprocalcitonin of mice with other species revealed highest homologies to the rat (93%) and human (77%) sequences. A recombinant form of mouse precalcitonin (rmPCT) of approximately 17 kDa was expressed as a fusion peptide in E.coli transformed with a PCR-cloned expression construct. |
