| First Author | Savini E | Year | 1994 |
| Journal | Gene | Volume | 144 |
| Issue | 2 | Pages | 253-7 |
| PubMed ID | 8039710 | Mgi Jnum | J:19305 |
| Mgi Id | MGI:67482 | Doi | 10.1016/0378-1119(94)90386-7 |
| Citation | Savini E, et al. (1994) Cloning and sequence analysis of a cDNA coding for the murine DNA ligase I enzyme. Gene 144(2):253-7 |
| abstractText | A complementary DNA (2961 bp) containing the complete coding sequence for murine DNA ligase I was isolated from a mouse fibroblast cDNA library using a cDNA encoding the human protein as a probe. An open reading frame of 2748 bp, encoding a protein of 916 amino acids (aa), was identified. Northern blot analysis of total RNA extracted from mouse fibroblasts showed a single band with a mobility corresponding to a size of 3.2 kb whose level increases upon serum stimulation of quiescent mouse NIH-3T3 cells. Alignment of the murine and human deduced aa sequences showed an overall 83% identity, that rises to 91% if only the sequence on the C-terminal portion of the protein containing the active site is considered. |
