| First Author | Maeda T | Year | 2005 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 46 |
| Issue | 11 | Pages | 4320-7 |
| PubMed ID | 16249514 | Mgi Jnum | J:103358 |
| Mgi Id | MGI:3609280 | Doi | 10.1167/iovs.05-0478 |
| Citation | Maeda T, et al. (2005) A critical role of CaBP4 in the cone synapse. Invest Ophthalmol Vis Sci 46(11):4320-7 |
| abstractText | PURPOSE: CaBP4, a photoreceptor-specific protein of the rods and cones, is essential for the development and maintenance of the mouse photoreceptor synapse. In this study, double CaBP4/rod alpha-transducin knockout (Cabp4(-/-)Gnat1(-/-)) mice lacking the rod-mediated component of electrophysiologic responses were generated and analyzed to investigate the role of CaBP4 in cones. METHODS: The retinal morphology and physiologic function of 2-month-old Cabp4(-/-)Gnat1(-/-) mice were analyzed using immunocytochemistry, electron microscopy, and single-flash and flicker electroretinography (ERG). RESULTS: The thickness of the outer plexiform layer and the number of photoreceptor terminals in Cabp4(-/-)Gnat1(-/-) mice were reduced to levels similar to those of Cabp4(-/-) mice. Single-flash and flicker ERG showed that the amplitude and sensitivity of the b-wave in the Cabp4(-/-)Gnat1(-/-) mice were severely attenuated compared with those in wild-type and Gnat1(-/-) mice. CONCLUSIONS: Results indicate that the cone synaptic function in Cabp4(-/-)Gnat1(-/-) mice was severely disrupted, whereas the morphologic defects observed in Cabp4(-/-)Gnat1(-/-) mice were similar to those of single Cabp4(-/-) knockout mice. This and a previous study reveal that CaBP4 is critical for signal transmission from rods and cones to second-order neurons. |
