| First Author | Ying H | Year | 2001 |
| Journal | Biochem Biophys Res Commun | Volume | 286 |
| Issue | 2 | Pages | 394-400 |
| PubMed ID | 11500051 | Mgi Jnum | J:71042 |
| Mgi Id | MGI:2149121 | Doi | 10.1006/bbrc.2001.5390 |
| Citation | Ying H, et al. (2001) Cloning and characterization of F-LANa, upregulated in human liver cancer. Biochem Biophys Res Commun 286(2):394-400 |
| abstractText | Differentially expressed genes between normal liver and hepatocellular carcinomas were investigated using differential display. Consequently, we identified a fragment cDNA upregulated in tumor tissues. We screened the liver library and cloned the full-length cDNA, named F-LANa. Increased expression of F-LANa was confirmed by Northern blot analysis in 10 of 14 (71%) cases of hepatocellular carcinomas. Human F-LANa gene maps to chromosome 17p at D17S1828-D17S786, spans at least 11.8 kb, and contains 7 exons. This gene encodes a 239 aa protein exhibiting 97.9% similarity to the mouse ortholog gene, identified later by in silico cloning. Homology analysis was carried out in various species and showed that F-LANa was evolutionarily conserved from yeast to human. In addition, F-LANa antisense oligonucleotide suppressed F-LANa expression in human hepatocellular carcinoma BEL-7404 cells and significantly inhibited cell growth. Together, our data demonstrate that overexpression of evolutionarily conserved F-LANa occurs frequently and may play an important role in proliferation. |
