| First Author | Tsuchihara K | Year | 2005 |
| Journal | Cancer Res | Volume | 65 |
| Issue | 15 | Pages | 6685-91 |
| PubMed ID | 16061649 | Mgi Jnum | J:100780 |
| Mgi Id | MGI:3589528 | Doi | 10.1158/0008-5472.CAN-04-4223 |
| Citation | Tsuchihara K, et al. (2005) Ckap2 regulates aneuploidy, cell cycling, and cell death in a p53-dependent manner. Cancer Res 65(15):6685-91 |
| abstractText | We used DNA microarray screening to identify Ckap2 (cytoskeleton associated protein 2) as a novel p53 target gene in a mouse erythroleukemia cell line. DNA damage induces human and mouse CKAP2 expression in a p53-dependent manner and p53 activates the Ckap2 promoter. Overexpressed Ckap2 colocalizes with and stabilizes microtubules. In p53-null cells, overexpression of Ckap2 induces tetraploidy with aberrant centrosome numbers, suggesting disturbed mitosis and cytokinesis. In p53-competent cells, Ckap2 does not induce tetraploidy but activates p53-mediated cell cycle arrest and apoptosis. Our data suggest the existence of a functional positive feedback loop in which Ckap2 activates the G1 tetraploidy checkpoint and prevents aneuploidy. |
