| First Author | Schatz DG | Year | 1989 |
| Journal | Cell | Volume | 59 |
| Issue | 6 | Pages | 1035-48 |
| PubMed ID | 2598259 | Mgi Jnum | J:10163 |
| Mgi Id | MGI:58619 | Doi | 10.1016/0092-8674(89)90760-5 |
| Citation | Schatz DG, et al. (1989) The V(D)J recombination activating gene, RAG-1. Cell 59(6):1035-48 |
| abstractText | The RAG-1 (recombination activating gene-1) genomic locus, which activates V(D)J recombination when introduced into NIH 3T3 fibroblasts, was isolated by serial genomic transfections of oligonucleotide-tagged DNA. A genomic walk spanning 55 kb yielded a RAG-1 genomic probe that detects a single 6.6-7.0 kb mRNA species in transfectants and pre-B and pre-T cells. RAG-1 genomic and cDNA clones were biologically active when introduced into NIH 3T3 cells. Nucleotide sequencing of human and mouse RAG-1 cDNA clones predicts 119 kd proteins of 1043 and 1040 amino acids, respectively, with 90% sequence identity. RAG-1 has been conserved between species that carry out V(D)J recombination, and its pattern of expression correlates exactly with the pattern of expression of V(D)J recombinase activity. RAG-1 may activate V(D)J recombination indirectly, or it may encode the V(D)J recombinase itself. |
