| First Author | Scott LM | Year | 1996 |
| Journal | Blood | Volume | 88 |
| Issue | 7 | Pages | 2517-30 |
| PubMed ID | 8839844 | Mgi Jnum | J:35784 |
| Mgi Id | MGI:83229 | Doi | 10.1182/blood.v88.7.2517.bloodjournal8872517 |
| Citation | Scott LM, et al. (1996) E3, a hematopoietic-specific transcript directly regulated by the retinoic acid receptor alpha. Blood 88(7):2517-30 |
| abstractText | Retinoic acid (RA)-induced maturation mediated by the retinoic acid receptor alpha (RAR alpha) has been implicated in myeloid development. We have used differential hybridization analysis of a cDNA library constructed from the murine RA-inducible MPRO promyelocyte cell line to identify immediate-early genes induced by RA during granulocytic differentiation. E3, one of nine sequences identified, was upregulated in an immediate-early manner, with transcript levels peaking after 60 minutes exposure to RA. E3 transcripts were RA-inducible in HL60 cells, but not in an RA-resistant subclone, HL60R, that harbors a mutated RAR alpha gene. However, when HL60R cells were transduced with a functional copy of the RAR alpha gene, RA induced a 10-fold increase in E3 mRNA levels. E3 transcripts are present in the myeloid, B-lymphoid, and erythroid lineages, absent in nonhematopoietic cells, and encode a highly hydrophobic, potentially phosphorylated polypeptide of unknown function with significant homology to a putative protein expressed in myeloid cells. The murine E3 promoter harbors a single bipartite retinoic acid response element which in transient transfection assays conferred RA sensitivity. These results indicate that E3 is a hematopoietic-specific gene that is an immediate target for the activated RAR alpha during myelopoiesis. |
