| First Author | Moss SB | Year | 1989 |
| Journal | Dev Biol | Volume | 133 |
| Issue | 1 | Pages | 83-92 |
| PubMed ID | 2707490 | Mgi Jnum | J:34257 |
| Mgi Id | MGI:81722 | Doi | 10.1016/0012-1606(89)90299-6 |
| Citation | Moss SB, et al. (1989) Expression of a novel histone 2B during mouse spermiogenesis. Dev Biol 133(1):83-92 |
| abstractText | During mammalian spermiogenesis transitional proteins and protamines replace histones on the DNA as the chromatin condenses. While previous studies suggested that histone genes are inactive postmeiotically, we have shown both by steady-state RNA analysis and nuclear run-off transcription assays that histone 2b (H2b) transcription occurs in mouse round spermatids. In addition, a novel H2b cDNA clone has been isolated from an adult mouse testes cDNA library. The sequence of this cDNA clone predicts a protein that is extremely similar to other mouse H2b proteins, except at the carboxyl-terminus where the testes H2b contains an additional 12 amino acids, seven of which are hydrophobic. In contrast to the replication-dependent histone mRNAs, the 3' untranslated region of this cDNA contains the poly(A) addition sequence (AAUAAA) upstream of a poly(A) tract. Furthermore, the conserved hairpin structure immediately upstream of replication-dependent histone mRNA termini is not present. Northern blot analysis of RNA from embryonic, ovarian, spermatogenic, and a variety of somatic tissues reveals that this novel H2b transcript is spermatid specific. The H2b mRNA is in polyribosomes isolated from spermatogenic cells, strongly suggesting that it is translated during spermiogenesis. |
