| First Author | Marchese A | Year | 1998 |
| Journal | Genomics | Volume | 50 |
| Issue | 2 | Pages | 281-6 |
| PubMed ID | 9653656 | Mgi Jnum | J:48334 |
| Mgi Id | MGI:1267184 | Doi | 10.1006/geno.1998.5297 |
| Citation | Marchese A, et al. (1998) Cloning genes encoding receptors related to chemoattractant receptors. Genomics 50(2):281-6 |
| abstractText | We report the cloning of a novel human gene (GPR32) encoding a putative G-protein-coupled receptor (GPCR) of 356 amino acids and a related pseudogene psi GPR32. The deduced amino acid sequence of GPR32 shares 35-39% identity with members of the chemoattractant receptor family. psi GPR32 shares 93% nucleotide identity with GPR32. We identified a mouse EST encoding a putative GPCR (GPR33) of 309 amino acids. The deduced amino acid sequence of GPR33 shares 30-35% identity with members of the chemoattractant receptor family and 36% identity with the receptor encoded by GPR32. The human orthologue of GPR33 contains a single basepair substitution with respect to the mouse, resulting in the presence of an in-frame stop codon within the predicted second intracellular loop, demonstrating that it is a pseudogene. Through fluorescence in situ hybridization and physical mapping of YACs, both GPR32 and psi GPR32 were mapped to chromosomal 19, region q13.3, while psi GPR33 was mapped to chromosome 14q12. |
