| First Author | Kone BC | Year | 1995 |
| Journal | Am J Physiol | Volume | 269 |
| Issue | 5 Pt 2 | Pages | F718-29 |
| PubMed ID | 7503239 | Mgi Jnum | J:39499 |
| Mgi Id | MGI:86893 | Doi | 10.1152/ajprenal.1995.269.5.F718 |
| Citation | Kone BC, et al. (1995) Role of NF-kappa B in the regulation of inducible nitric oxide synthase in an MTAL cell line. Am J Physiol 269(5 Pt 2):F718-29 |
| abstractText | The effects of cytokines, lipopolysaccharide (LPS), 8-bromoadenosine 3-prime,5-prime-cyclic monophosphate (8-BrcAMP), and pyrrolidine dithiocarbamate (PDTC), an inhibitor of nuclear factor kappa B (NF-kappa B) activation, on inducible nitric oxide synthase (iNOS) expression were studied in the medullary thick ascending limb of Henles loop cell line ST-1. LPS + interferon-gamma (IF-gamma) promoted a time-dependent increase in nitrite (a NO metabolite) and iNOS mRNA and the appearance of NF-kappa B p50 and p65 in nuclear protein extracts. Actinomycin D but not cycloheximide prevented the LPS + IF-gamma induction of iNOS mRNA and NO synthesis, indicating that iNOS transcriptional activation by LPS + IF-gamma does not require newly synthesized proteins. PDTC inhibited the LPS + IF-gamma induction of NO, iNOS mRNA, and the appearance of NF-kappa B in nuclear protein extracts, suggesting that NF-kappa B mobilization and trans-activation of the iNOS gene mediates this induction. In contrast to other cell types, cycloheximide did not alter iNOS mRNA stability, and 8-BrcAMP did not alter basal or LPS+IF-gamma induced NO production in ST-1 cells. |
