| First Author | Trieu M | Year | 1999 |
| Journal | J Neurosci | Volume | 19 |
| Issue | 15 | Pages | 6549-58 |
| PubMed ID | 10414983 | Mgi Jnum | J:56447 |
| Mgi Id | MGI:1340968 | Doi | 10.1523/JNEUROSCI.19-15-06549.1999 |
| Citation | Trieu M, et al. (1999) Autoregulatory sequences are revealed by complex stability screening of the mouse brn-3.0 locus. J Neurosci 19(15):6549-58 |
| abstractText | The POU-IV or Brn-3 class of transcription factors exhibit conserved structure, DNA-binding properties, and expression in specific subclasses of neurons across widely diverged species. In the mouse CNS, Brn-3.0 expression characterizes specific neurons from neurogenesis through the life of the cell. This irreversible activation of expression suggests positive autoregulation. To search for cis-acting elements that could mediate autoregulation we used a novel method, complex stability screening, which we applied to rapidly identify functional Brn-3.0 recognition sites within a large genomic region encompassing the mouse brn-3.0 locus. This method is based on the observation that the kinetic stability of Brn3.0 complexes with specific DNA sequences, as measured by their dissociation half-lives, is highly correlated with the ability of those sequences to mediate transcriptional activation by Brn- 3.0. The principal Brn-3.0 autoregulatory region lies similar to 5 kb upstream from the Brn-3.0 transcription start site and contains multiple Brn-3.0-binding sites that strongly resemble the optimal binding site for this protein class. This region also mediates transactivation by the closely related protein Brn-3.2, suggesting a regulatory cascade of POU proteins in specific neurons in which Brn-3.2 expression precedes Brn-3.0. |
