| First Author | Campbell HD | Year | 1985 |
| Journal | Eur J Biochem | Volume | 150 |
| Issue | 2 | Pages | 297-304 |
| PubMed ID | 3926497 | Mgi Jnum | J:7930 |
| Mgi Id | MGI:56399 | Doi | 10.1111/j.1432-1033.1985.tb09020.x |
| Citation | Campbell HD, et al. (1985) Cloning and nucleotide sequence of the murine interleukin-3 gene. Eur J Biochem 150(2):297-304 |
| abstractText | Southern hybridization analysis using a probe derived from a murine interleukin-3 (IL-3) cDNA clone revealed the presence of a single IL-3 gene in the haploid murine genome. An 8600-base-pair (8.6-kb) murine genomic EcoRI fragment containing the IL-3 gene was isolated by screening a library of size-fractionated genomic EcoRI fragments cloned in lambda gtWES X lambda B. The nucleotide sequence of a 3.5-kb region of the cloned DNA encompassing the IL-3 gene was determined. The gene contains four introns of 96, 993, 135 and 122 base pairs (bp), located within the coding region. The large intron contains 12 copies of a 14-15-bp tandem repeating sequence which resembles a human cellular homologue of a BKV enhancer sequence. The nucleotide sequence of the exons agrees exactly with that of an IL-3 cDNA cloned from WEHI-3, a tumorigenic cell line which over-produces IL-3, establishing that the unprocessed primary structure of IL-3 is identical in WEHI-3 and in BALB/c mice. Southern hybridization has revealed genomic alteration in the vicinity of the IL-3 gene in WEHI-3 cells. |
