| First Author | Foulkes NS | Year | 1991 |
| Journal | Cell | Volume | 64 |
| Issue | 4 | Pages | 739-49 |
| PubMed ID | 1847666 | Mgi Jnum | J:18605 |
| Mgi Id | MGI:66867 | Doi | 10.1016/0092-8674(91)90503-q |
| Citation | Foulkes NS, et al. (1991) CREM gene: use of alternative DNA-binding domains generates multiple antagonists of cAMP-induced transcription. Cell 64(4):739-49 |
| abstractText | We isolated a gene from a mouse pituitary cDNA library that encodes a protein highly homologous to nuclear factor CREB, an activator of cAMP-responsive promoter elements (CREs). We demonstrate that while CREB is expressed uniformly in several cell types, this gene, termed CREM, shows cell-specific expression. CREM has a remarkable organization, since down-stream of the stop codon there is a second, out-of-frame DNA-binding domain. Using PCR and RNAase protection analysis, we have identified three mRNA isoforms that appear to be obtained by differential cell-specific splicing. Sequencing of the isoforms demonstrated alternative usage of the two DNA-binding domains. CREM proteins reveal the same efficiency and specificity of binding to CRE sequences as CREB, but in contrast to CREB, CREM acts as a down-regulator of cAMP-induced transcription. |
