| First Author | Fathi Z | Year | 1995 |
| Journal | Neurochem Res | Volume | 20 |
| Issue | 1 | Pages | 107-13 |
| PubMed ID | 7739752 | Mgi Jnum | J:26939 |
| Mgi Id | MGI:74363 | Doi | 10.1007/BF00995160 |
| Citation | Fathi Z, et al. (1995) Cloning, expression, and tissue distribution of a fifth melanocortin receptor subtype. Neurochem Res 20(1):107-13 |
| abstractText | The melanocortin (MC) peptides mediate a diverse spectrum of biological activities in both the central nervous system and peripheral tissues by interacting with specific guanine nucleotide binding (G protein)-coupled receptors. Previously, four human melanocortin receptor subtypes have been cloned and characterized. In this study, we have isolated mouse complementary DNA (cDNA) and human genomic clones encoding a fifth melanocortin receptor subtype, MC5. Melanocortin peptide stimulation of human MC5, transiently expressed in COS1 cells, results in activation of adenylate cyclase with the following rank order of potency: [Nle4, D-Phe7]-alpha-MSH (melanocyte stimulating hormone) > ACTH (1-24) (adrenocorticotropic hormone) > alpha-MSH > beta-MSH > gamma-MSH. Northern blot hybridization, ribonuclease protection, and reverse transcription/polymerase chain reaction assays indicate that mouse MC5 mRNA is most abundant in skeletal muscle and brain. Lower but detectable levels of MC5 mRNA are also found in RT2-2 retinal neuronal cells, lung, testis, spleen, heart, kidney, and liver. |
