| First Author | Wang J | Year | 2008 |
| Journal | Mol Cell Biol | Volume | 28 |
| Issue | 7 | Pages | 2213-20 |
| PubMed ID | 18227153 | Mgi Jnum | J:134207 |
| Mgi Id | MGI:3785131 | Doi | 10.1128/MCB.01608-07 |
| Citation | Wang J, et al. (2008) Bifunctional role of Rev-erbalpha in adipocyte differentiation. Mol Cell Biol 28(7):2213-20 |
| abstractText | The nuclear receptor Rev-erbalpha is a potent transcriptional repressor that regulates circadian rhythm and metabolism. Here we demonstrate a dissociation between Rev-erbalpha mRNA and protein levels that profoundly influences adipocyte differentiation. During adipogenesis, Rev-erbalpha gene expression initially declines and subsequently increases. Remarkably, Rev-erbalpha protein levels are nearly the opposite, increasing early in adipogenesis and then markedly decreasing in adipocytes. The Rev-erbalpha protein is necessary for the early mitotic events that are required for adipogenesis. The subsequent reduction in Rev-erbalpha protein, due to increased degradation via the 26S proteasome, is also required for adipocyte differentiation because Rev-erbalpha represses the expression of PPARgamma2, the master transcriptional regulator of adipogenesis. Thus, opposite to what might be predicted from Rev-erbalpha gene expression, Rev-erbalpha protein levels must rise and then fall for adipocyte differentiation to occur. |
