| First Author | Lurquin C | Year | 1989 |
| Journal | Cell | Volume | 58 |
| Issue | 2 | Pages | 293-303 |
| PubMed ID | 2568889 | Mgi Jnum | J:9898 |
| Mgi Id | MGI:58355 | Doi | 10.1016/0092-8674(89)90844-1 |
| Citation | Lurquin C, et al. (1989) Structure of the gene of tum- transplantation antigen P91A: the mutated exon encodes a peptide recognized with Ld by cytolytic T cells. Cell 58(2):293-303 |
| abstractText | Mutagen treatment of mouse P815 tumor cells produces immunogenic mutants that express new transplantation antigens (tum- antigens) recognized by cytolytic T cells. We found that the gene conferring expression of tum- antigen P91A contains 12 exons, encoding a 60 kd protein lacking a typical N-terminal signal sequence. The sequence shows no significant similarity with sequences in current data bases. A mutation that causes expression of the antigen is located in exon 4; it is the only apparent difference between the normal and the antigenic alleles. A short synthetic peptide corresponding to a region of exon 4 located around this mutation makes P815 cells sensitive to lysis by anti-P91A cytolytic T cells. The mutation creates a strong aggretope enabling the peptide to bind the H-2 Ld molecule. Several secondary tumor cell variants that no longer express tum- antigen P91A were found to carry deletions in the gene. |
