| First Author | Tanaka H | Year | 1993 |
| Journal | Biochem Biophys Res Commun | Volume | 190 |
| Issue | 3 | Pages | 732-40 |
| PubMed ID | 8382489 | Mgi Jnum | J:3961 |
| Mgi Id | MGI:52466 | Doi | 10.1006/bbrc.1993.1110 |
| Citation | Tanaka H, et al. (1993) Molecular cloning and expression of the mouse 105-kDa gelatinase cDNA. Biochem Biophys Res Commun 190(3):732-40 |
| abstractText | We have detected a potent gelatinolytic activity in the culture supernatant of a metastatic tumor line, SN-H, derived from a murine squamous cell carcinoma. The relative molecular weight of the gelatinase was estimated as 105-kDa by gelatin zymography. We have cloned the cDNA of this gelatinase and the 3160-bp sequence has been determined. From the translated amino acid sequence, the positions of the cysteine residues and the functional domain structure are highly homologous to the human 92-kDa gelatinase. The nucleotide and amino acid sequence homology between these two cDNAs are 75% and 72%, respectively. Transfection of the cDNA in an expression vector resulted in production of the 105-kDa gelatinase, thus confirming that this cDNA is functional. |
