| First Author | Jiao K | Year | 2002 |
| Journal | Mol Cell Biol | Volume | 22 |
| Issue | 21 | Pages | 7633-44 |
| PubMed ID | 12370310 | Mgi Jnum | J:79484 |
| Mgi Id | MGI:2388376 | Doi | 10.1128/MCB.22.21.7633-7644.2002 |
| Citation | Jiao K, et al. (2002) Identification of mZnf8, a Mouse Kruppel-Like Transcriptional Repressor, as a Novel Nuclear Interaction Partner of Smad1. Mol Cell Biol 22(21):7633-44 |
| abstractText | To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Kruppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with various in vitro and in vivo approaches, including mammalian two-hybrid, in vitro glutathione S-transferase pulldown, and copurification assays. Results from functional analysis indicate that mZnf8 is a nuclear transcriptional repressor. Overexpression of mZnf8 represses activity of BMP and transforming growth factor beta (TGF-beta) reporters. Silencing the expression of endogenous mZnf8 with an RNA interference approach caused a significant increase in the expression of one BMP reporter. These results suggest that mZnf8 negatively regulates the TGF-beta/BMP signaling pathway in vivo. Transcription of mZnf8 is ubiquitous in mouse embryos, but high levels are specifically observed in adult mouse testes, with the same cell- and stage-specific transcription pattern as Smad1. Our data support the hypothesis that mZnf8 plays critical roles in mediating BMP signaling during spermatogenesis. |
