| First Author | Fisicaro N | Year | 1995 |
| Journal | Mol Immunol | Volume | 32 |
| Issue | 8 | Pages | 565-72 |
| PubMed ID | 7609734 | Mgi Jnum | J:26700 |
| Mgi Id | MGI:74133 | Doi | 10.1016/0161-5890(95)00032-a |
| Citation | Fisicaro N, et al. (1995) Identification of genes downregulated in the thymus by cyclosporin-A: preliminary characterization of clone CSA-19. Mol Immunol 32(8):565-72 |
| abstractText | Using a novel subtractive hybridization approach, we have identified a set of cDNA clones whose expression is downregulated in the thymus by cyclosporin-A (CsA). A number of regulated genes were identified, but the major focus of this report is the clone termed CsA-19. In the adult mouse, CsA-19 is expressed at high levels in lymphoid organs, particularly in secondary lymphoid organs such as lymph node and spleen. CsA-19 expression was also found to be regulated in the brain and liver during mouse development. The full length sequences of the murine and human CsA-19 cDNAs have been determined. Both are 700 nucleotides in length and encode a predicted product of 217 amino acids. Comparison of the full length cDNA sequences of murine and human CsA-19 reveal that they are greater than 91% conserved. At the predicted amino acid level the homology is even greater, with only two amino acid differences in 217 residues (99% identity). The high degree of homology between murine and human CsA-19 indicates that has there been strong evolutionary pressure to conserve the amino acid sequence of CsA-19, and suggests that CsA-19 may play a critical role both during embryogenesis and in mature lymphoid cells. |
