| First Author | Schumacher JM | Year | 1995 |
| Journal | J Cell Biol | Volume | 129 |
| Issue | 4 | Pages | 1023-32 |
| PubMed ID | 7744952 | Mgi Jnum | J:25219 |
| Mgi Id | MGI:72947 | Doi | 10.1083/jcb.129.4.1023 |
| Citation | Schumacher JM, et al. (1995) Spnr, a murine RNA-binding protein that is localized to cytoplasmic microtubules. J Cell Biol 129(4):1023-32 |
| abstractText | Previous studies in transgenic mice have established the importance of the 3' untranslated region (UTR) of the spermatid-specific protamine-1 (Prm-1) mRNA in its translational control during male germ cell development. To clone genes that mediate the translational repression or activation of the Prm-1 mRNA, we screened cDNA expression libraries made with RNA from pachytene spermatocytes and round spermatids, with an RNA probe corresponding to the 3' UTR of Prm-1. We obtained six independent clones that encode Spnr, a spermatid perinuclear RNA-binding protein. Spnr is a 71-kD protein that contains two previously described RNA binding domains. The Spnr mRNA is expressed at high levels in the testis, ovary, and brain, and is present in multiple forms in those tissues. Immunolocalization of the Spnr protein within the testis shows that it is expressed exclusively in postmeiotic germ cells and that it is localized to the manchette, a spermatid-specific microtubular array. Although the Spnr protein is expressed too late to be directly involved in the translational repression of Prm-1 specifically, we suggest that the Spnr protein may be involved in other aspects of spermatid RNA metabolism, such as RNA transport or translational activation. |
