| First Author | Benoit De Coignac A | Year | 1998 |
| Journal | Gene | Volume | 209 |
| Issue | 1-2 | Pages | 149-56 |
| PubMed ID | 9524254 | Mgi Jnum | J:46859 |
| Mgi Id | MGI:1202153 | Doi | 10.1016/s0378-1119(98)00040-7 |
| Citation | Benoit De Coignac A, et al. (1998) cDNA cloning and expression analysis of the murine ribonuclease L inhibitor. Gene 209(1-2):149-56 |
| abstractText | The 2-5A/RNase L system is one of the pathways induced by interferon (IFN). It plays a major role in the antiviral and antiproliferative activities of IFNs. Recently, we have shown that the activity of the RNase L could be inhibited by a proteic inhibitor, the RNase L Inhibitor (RLI). Human RLI (Hu-RLI) was cloned and characterized. We describe here the isolation and characterization of the cDNA encoding the murine RLI (Mu-RLI). Hu-RLI and Mu-RLI protein have 98% amino acid identity. Mu-RLI is functionally homologous to Hu-RLI, and all the structural features and amino acid sequence motifs of Hu-RLI are conserved in Mu-RLI. Moreover, reticulocyte lysate translated Mu-RLI protein is also able to inhibit 2-5A binding on 2-5A-dependent RNAse-L. Northern blot analysis revealed that Mu-RLI cDNA hybridizes with one mRNA of 3.5 kb except for the testis where two mRNA of 3.5 and 2.1 kb, respectively, are detected, suggesting a tissue-specific regulation. |
