| First Author | Hasegawa T | Year | 1997 |
| Journal | J Biol Chem | Volume | 272 |
| Issue | 8 | Pages | 4915-23 |
| PubMed ID | 9030551 | Mgi Jnum | J:38556 |
| Mgi Id | MGI:85937 | Doi | 10.1074/jbc.272.8.4915 |
| Citation | Hasegawa T, et al. (1997) Cloning and characterization of a transcription factor that binds to the proximal promoters of the two mouse type I collagen genes. J Biol Chem 272(8):4915-23 |
| abstractText | We have used the yeast one-hybrid system to clone transcription factors that bind to specific sequences in the proximal promoters of the type I collagen genes. We utilized as bait the sequence between -180 and -136 in the pro-alpha2(I) collagen promoter because it acts as a functional promoter element and binds several DNA-binding proteins. Three cDNA clones were isolated that encoded portions of the mouse SPR2 transcription factor, whereas a fourth cDNA contained a potential open reading frame for a polypeptide of 775 amino acids and was designated BFCOL1. Recombinant BFCOL1 was shown to bind to the -180 to -152 segment of the mouse pro-alpha2(I) collagen proximal promoter and to two discrete sites in the proximal promoter of the mouse pro-alpha1(I) gene. The N-terminal portion of BFCOL1 contains its DNA-binding domain. DNA transfection experiments using fusion polypeptides with the yeast GAL4 DNA-binding segment indicated that the C-terminal part of BFCOL1 contained a potential transcriptional activation domain. We speculate that BFCOL1 participates in the transcriptional control of the two type I collagen genes. |
