First Author | Chen L | Year | 1998 |
Journal | DNA Seq | Volume | 9 |
Issue | 2 | Pages | 101-8 |
PubMed ID | 10520738 | Mgi Jnum | J:55252 |
Mgi Id | MGI:1337545 | Doi | 10.3109/10425179809086434 |
Citation | Chen L, et al. (1998) Molecular cloning of a leucine zipper motif-containing novel cDNA specifically expressed in adult mouse testis. DNA Seq 9(2):101-8 |
abstractText | A novel cDNA clone was isolated from a mouse testis cDNA library. This cDNA is 2,020 nucleotides in size and predicted to encode 527 amino acid residues with a molecular weight of 59.9 kDa. Protein sequence motif analysis revealed that the predicted protein contained one leucine zipper motif in its N-terminal portion and two basic domains in its C-terminal portion. Northern blot analysis of adult ICR mouse organs using the cDNA as probe demonstrated that an approximately 2.0-kb transcript was specifically expressed in testis. We therefore termed this novel cDNA tsec-2, testis-specifically expressed cDNAs-2. Results of Southern blot analysis suggested that the tsec-2 gene may exist as a single copy in the mouse genome. |