| First Author | Roesch K | Year | 2008 |
| Journal | J Comp Neurol | Volume | 509 |
| Issue | 2 | Pages | 225-38 |
| PubMed ID | 18465787 | Mgi Jnum | J:165545 |
| Mgi Id | MGI:4837737 | Doi | 10.1002/cne.21730 |
| Citation | Roesch K, et al. (2008) The transcriptome of retinal Muller glial cells. J Comp Neurol 509(2):225-38 |
| abstractText | Muller glial cells are the major type of glia in the mammalian retina. To identify the molecular machinery that defines Muller glial cell identity and function, single cell gene expression profiling was performed on Affymetrix microarrays. Identification of a cluster of genes expressed at high levels suggests a Muller glia core transcriptome, which likely underlies many of the functions of Muller glia. Expression of components of the cell cycle machinery and the Notch pathway, as well as of growth factors, chemokines, and lipoproteins might allow communication between Muller glial cells and the neurons that they support, including modulation of neuronal activity. This approach revealed a set of transcripts that were not previously characterized in (Muller) glia; validation of the expression of some of these genes was performed by in situ hybridization. Genes expressed exclusively by Muller glia were identified as novel markers. In addition, a novel BAC transgenic mouse that expresses Cre in Muller glia cells was generated. The molecular fingerprint of Muller glia provides a foundation for further studies of Muller glia development and function in normal and diseased states. |
