| First Author | Lin AW | Year | 2018 |
| Journal | Life Sci Alliance | Volume | 1 |
| Issue | 6 | Pages | e201800118 |
| PubMed ID | 30623173 | Mgi Jnum | J:277303 |
| Mgi Id | MGI:6330864 | Doi | 10.26508/lsa.201800118 |
| Citation | Lin AW, et al. (2018) Chemical genetic identification of GAK substrates reveals its role in regulating Na(+)/K(+)-ATPase. Life Sci Alliance 1(6):e201800118 |
| abstractText | Cyclin G-associated kinase (GAK) is a ubiquitous serine/threonine kinase that facilitates clathrin uncoating during vesicle trafficking. GAK phosphorylates a coat adaptor component, AP2M1, to help achieve this function. GAK is also implicated in Parkinson's disease through genome-wide association studies. However, GAK's role in mammalian neurons remains unclear, and insight may come from identification of further substrates. Employing a chemical genetics method, we show here that the sodium potassium pump (Na(+)/K(+)-ATPase) alpha-subunit Atp1a3 is a GAK target and that GAK regulates Na(+)/K(+)-ATPase trafficking to the plasma membrane. Whole-cell patch clamp recordings from CA1 pyramidal neurons in GAK conditional knockout mice show a larger change in resting membrane potential when exposed to the Na(+)/K(+)-ATPase blocker ouabain, indicating compromised Na(+)/K(+)-ATPase function in GAK knockouts. Our results suggest a modulatory role for GAK via phosphoregulation of substrates such as Atp1a3 during cargo trafficking. |
