| First Author | Borges AC | Year | 2002 |
| Journal | Int J Dev Biol | Volume | 46 |
| Issue | 2 | Pages | 259-62 |
| PubMed ID | 11934155 | Mgi Jnum | J:79941 |
| Mgi Id | MGI:2389604 | Doi | 10.1387/ijdb.011496 |
| Citation | Borges AC, et al. (2002) Goosecoid and cerberus-like do not interact during mouse embryogenesis. Int J Dev Biol 46(2):259-62 |
| abstractText | Mouse Cerberus-like (Cer-l) is a neural inducer molecule, capable of inhibiting Nodal and BMP-4 signals in the extracelular space. The cer-l expression domain in the Anterior Visceral Endoderm (AVE) and prechordal plate, tissues involved in head induction and patterning, respectively, suggested a role for this gene in head formation. However, animals homozygous for the cer-l null allele failed to show any abnormality, leading us to propose the existence of other factor(s) that might compensate for cer-l loss-of-function. Since goosecoid (gsc) shares some domains of expression with cer-l and was shown to be essential for head morphogenesis, we tested its ability to interact genetically with cer-l. With this aim we generated cer-l;gsc double mutants. These animals were analyzed at birth for skeletal defects and revealed the same phenotype as gsc-/- single mutants. We also investigated the proper patterning of structures adjacent to the prechordal plate by performing in situ hybridization of HNF-3beta, Six-3 and BF-1, genes whose expression domains remained unchanged. In conclusion, the analysis carried out indicated that gsc does not compensate for cer-l loss-of-function and that these genes do not interact genetically. |
