| Primary Identifier | MGI:2154520 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cdk4 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked neomycin resistance cassette was inserted between exons 1 and 2. In addition, the AAGCCCGA sequence in exon 2 (nt 81-88) was replaced by AAGCTTGT. This alters codon 24 from one corresponding to arginine to a cysteine. A third silent substitution at position nt85 was used to create a diagnostic restriction enzyme site to monitor the presence of the mutation. RT-PCR analysis revealed that no transcript was produced from this allele in testis, ovaries or pancreas. Western blot analysis demonstrated that no protein was detectable in fibroblasts isolated from E14.5 homozygous embryos. |
