| Primary Identifier | MGI:6704921 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cacna1i |
| Strain of Origin | Not Specified | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology using sgRNA 5'-TCAGCCTCCGCACGGACAC-3' generated a 10 bp deletion (TCCCTGACAG) beginning at the codon corresponding to amino acid V756 resulting in a premature stop codon. Western blot analysis confirmed the absence of protein in homozygous brain. |
