| First Author | Siddiqui IA | Year | 2008 |
| Journal | Pharm Res | Volume | 25 |
| Issue | 9 | Pages | 2135-42 |
| PubMed ID | 18317887 | Mgi Jnum | J:138094 |
| Mgi Id | MGI:3804161 | Doi | 10.1007/s11095-008-9553-z |
| Citation | Siddiqui IA, et al. (2008) Suppression of NFkappaB and its Regulated Gene Products by Oral Administration of Green Tea Polyphenols in an Autochthonous Mouse Prostate Cancer Model. Pharm Res 25(9):2135-42 |
| abstractText | PURPOSE: This study examines the role of cell survival/apoptosis related proteins involved in NFkappaB signaling pathways and its associated events in GTP-induced chemoprevention of prostate cancer in TRAMP mice. METHODS: Mice were given 0.1% GTP as drinking fluid. Western blot and immunohistochemical analysis performed to examine NFkappaB and its regulated pathway in response to GTP. RESULTS: Our data demonstrated increased expression of NFkappaB, IKKalpha, IKKbeta, RANK, NIK and STAT-3 in dorso-lateral prostate of TRAMP mice as a function of age and tumor growth and continuous GTP infusion for 32 weeks resulted in substantial reduction in these proteins. The levels of transcription factor osteopontin, a non-collagenous extracellular matrix protein, were also downregulated. Inhibition of NFkappaB signaling is known to activate apoptotic and inhibit anti-apoptotic proteins. Therefore, we analyzed Bax and Bcl(2) levels in the dorsolateral prostate of TRAMP mice fed GTP and observed a shift in balance between Bax and Bcl(2) favoring apoptosis. CONCLUSIONS: Based on the data we suggest that oral consumption of GTP might inhibit osteopontin and NFkappaB signaling that may contribute to induction of apoptosis observed in GTP fed TRAMP mice. |
