Primary Identifier | MGI:5300514 | Allele Type | Transgenic |
Attribute String | Transposase | Gene | Tg(PGK2-sb10)1780Ove |
Strain of Origin | FVB/N | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The 3.3 kbp PGK2-SB10-SV40 transgene was designed with the 1.4 kbp human phosphoglycerol kinase 2 promoter (PGK2) sequence, the coding sequence for the SB10 Sleeping Beauty transposase, and the SV40 early region intron/polyA signal. The SB10 gene is a majority-rule consensus sequence (engineered to eliminate the inactivating mutations from a Tc1-like element transposase gene of the salmonid subfamily) encoding the 340 amino acid synthetic, putative transposase gene. Line 1780 was generated. |