| Primary Identifier | MGI:6195846 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Pfdn1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 4 guide sequences TCACGGGGCTCTCTTAGGAG, GTAGCCAGAGAGTAATAAAA, TCTCACTCTCTGTCGGGGCA and GGTCATGCGGTCTATGGATA, which resulted in a 493 bp deletion beginning at Chromosome 18 position 36,451,035 bp and ending after 36,451,527 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000141136 (exon 2) and 326 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 11 and early truncation 24 amino acids later. |
