| Primary Identifier | MGI:3811558 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Gjb4 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A lacZ gene with a nuclear localization signal followed by a frt-flanked neomycin selection cassette in the reverse transcriptional orientation was inserted into the starting codon of the gene locus. Founder mice were crossed with a FLP-deleter strain to remove the neomycin selection cassette. Gene inactivation was confirmed by immunoblot analysis of skin lysates. LacZ expression was detected in the skin, kidney, and a subpopulation of cells in the apical layer of the vomeronasal organ. |
