Primary Identifier | MGI:5908450 | Allele Type | Targeted |
Attribute String | Inducible, Null/knockout, Recombinase | Gene | Postn |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | Tamoxifen-inducible cre recombinase (MerCreMer: cre recombinase fused to two modified estrogen receptor ligand binding domains at both ends) cDNA was introduced, in-frame, to the ATG start site of exon 1 via homologous recombination. The plasmid also contains a diptheria toxi A cDNA and a FRT-flanked neomycin cassette for negative and positive selection. Flp-mediated recombination removed the FRT-flanked neo cassette. |