| Primary Identifier | MGI:3052969 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(pPWL512hyg)1Ems |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| description | Transgene also called "feeder" and "hyg." Transgenic mice on a C57BL/6 background express the hygromycin resistance gene in a wide variety of tissues including muscle. Embryonic feeder layers derived from these mice are resistant to up to 0.8mg/ml hygromycin B, whereas feeder layers from normal C57BL/6J mice are killed by 0.2mg/ml hygromycin B. Mouse embryonic fibroblasts prepared from homozygous transgenic mice that are also homozygous for Tg(Hprt1)1Jae and Dnmt1tm3Jae are resistant to concentrations of the drugs G418, 6TG, puromycin, and hygromycin well above those used normally for the selection of drug-resistant ES cells. |
| molecularNote | The transgene contains a PGK promoter and the bacterial hygromycin resistance gene. |
