| Primary Identifier | MGI:6294062 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Myh6-COP4*L132C,-lacZ)B5-7Mik |
| Strain of Origin | FVB/N X B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The transgene is designed with (from 5' to 3') a ~5.5 kbp mouse Myh6 minimal promoter sequence (including the 3' most exon of betaMHC, the first three exons of alphaMHC [up to the start codon] as well as intervening sequences), a CatCh-IRES-lacZ-hGHpA construct (CatCh, internal ribosome entry site, beta-galactosidase and a human growth hormone polyadenylation signal). The calcium translocating channelrhodopsin CatCh [ChR2(L132C)] cDNA sequence used encodes the first 309 amino acids of green alga Chlamydomonas reinhardtii channelrhodopsin-2 (ChR2 or cop4) that has been modified with an L132C amino acid substitution (CTC-to-TGC) designed to cause enhanced Ca2+ permeability. Founder line B5-7 was identified with high sensor expression in cardiomyocytes. |
