| First Author | Li L | Year | 2022 |
| Journal | Biochem Biophys Res Commun | Volume | 626 |
| Pages | 129-134 | PubMed ID | 35988296 |
| Mgi Jnum | J:328243 | Mgi Id | MGI:7335688 |
| Doi | 10.1016/j.bbrc.2022.07.084 | Citation | Li L, et al. (2022) Taurine promotes estrogen synthesis by regulating microRNA-7a2 in mice ovarian granulosa cells. Biochem Biophys Res Commun 626:129-134 |
| abstractText | Taurine, acting as a free amino acid, is widely distributed and plays multiple functions, including its regulating effect on estrogen synthesis in ovary. However, the mechanisms of taurine regulating estrogen synthesis in granulosa cells are not well understood. In this study, we identify whether microRNA-7a2 (miR-7a2) is involved in the signaling of taurine regulating estrogen synthesis in mouse granulosa cells for the first time. The results demonstrated that taurine transporter (TauT) co-localized with miR-7a in mouse ovarian granulose cells. Further, taurine treatment markedly enhanced the expression of miR-7a and Cyp19a1 in mouse ovaries and increased serum 17beta-estradiol (E2) concentration. Meanwhile, miR-7a2 knockout reversed the effect of taurine on E2. In addition, Golgi apparatus protein 1 (Glg1), a downstream target gene of miR-7a2, was significantly down-regulated by taurine, while Glg1 knockdown markedly increased the Cyp19a1 expression and E2 synthesis. Moreover, taurine affected miR-7a expression via activating p38 signaling. These results suggest that taurine promotes E2 synthesis through p38/miR-7a/Glg1/Cyp19a1 signaling pathway, which is crucial to understand the function and mechanism of taurine on estrogen synthesis. |
