| Primary Identifier | MGI:2388333 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Lck-ZAP70-Y315F)1Achn |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| description | Two transgenic founders, line 1059 and line 1075, expressing comparable amounts of transgene product were created. |
| molecularNote | The human ZAP70 gene carrying a tyrosine to phenylalanine substitution at position 315 (Y315F) was used as the transgene. The transgene is expressed in thymocytes under the control of mouse Lck promoter and contains a hemagglutinin epitope tag at the N-terminal end and a human growth hormone polyA tail. Transgene expression was verified by Western blot analysis. |
