| First Author | Park JH | Year | 2012 |
| Journal | J Neurochem | Volume | 122 |
| Issue | 5 | Pages | 1081-91 |
| PubMed ID | 22765017 | Mgi Jnum | J:187626 |
| Mgi Id | MGI:5437564 | Doi | 10.1111/j.1471-4159.2012.07861.x |
| Citation | Park JH, et al. (2012) Phosphorylation of Munc18-1 by Dyrk1A regulates its interaction with Syntaxin 1 and X11alpha. J Neurochem 122(5):1081-91 |
| abstractText | Dual-specificity tyrosine(Y)-phosphorylation-regulated kinase 1A (Dyrk1A) is a protein kinase that might be responsible for mental retardation and early onset of Alzheimer's disease in Down's syndrome patients. Dyrk1A plays a role in many cellular pathways through phosphorylation of diverse substrate proteins; however, its role in synaptic vesicle exocytosis is poorly understood. Munc18-1, a central regulator of neurotransmitter release, interacts with Syntaxin 1 and X11alpha. Syntaxin 1 is a key soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein involved in synaptic vesicle docking/fusion events, and X11alpha modulates amyloid precursor protein processing and beta amyloid generation. In this study, we demonstrate that Dyrk1A interacts with and phosphorylates Munc18-1 at the Thr(479) residue. The phosphorylation of Munc18-1 at Thr(479) by Dyrk1A stimulated binding of Munc18-1 to Syntaxin 1 and X11alpha. Furthermore, the levels of phospho-Thr(479) -Munc18-1 were enhanced in the brains of transgenic mice over-expressing Dyrk1A protein, providing in vivo evidence of Munc18-1 phosphorylation by Dyrk1A. These results reveal a link between Munc18-1 and Dyrk1A in synaptic vesicle trafficking and amyloid precursor protein processing, suggesting that up-regulated Dyrk1A in Down's syndrome and Alzheimer's disease brains may contribute to some pathological features, including synaptic dysfunction and cognitive defect through abnormal phosphorylation of Munc18-1. |
