| Primary Identifier | MGI:2386241 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence, Null/knockout | Gene | Gja1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The endogenous gene was deleted and replaced with Gja5 genomic sequence extending from 270 bp upstream of the start codon to 70 bp downstream of the stop codon. The transcription of Gja5 and the ablation of endogenous expression was shown by Northern blot analysis. The absence of endogenous protein was demonstrated by Western blot ananlysis of heart tissue, which also confirmed the presence of the Gja5 protein. |
