| Primary Identifier | MGI:3527191 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ppt1 |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 4, encoding the active site, was replaced with a loxP flanked hygromycin B resistance cassette. Tranfection of ES cells with a cre-expressing plasmid resulted in the excision of the hygro. Brain tissue from mutants was analyzed by RT-PCR to show the presence of the altered transcript. Sequence analysis revealed a frameshift and a premature stop codon after exon 3. |
